Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins-rabbit IgG antibody system by whole-column imaged capillary isoelectric focusing

Authors
Wu, XZ Huang, TM Mullett, WM Yeung, JM Pawliszyn, J
Citation
Xz. Wu et al., Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins-rabbit IgG antibody system by whole-column imaged capillary isoelectric focusing, J MICROCOL, 13(8), 2001, pp. 322-326
Citations number
20
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF MICROCOLUMN SEPARATIONS
ISSN journal
10407685 → ACNP
Volume
13
Issue
8
Year of publication
2001
Pages
322 - 326
Database
ISI
SICI code
1040-7685(2001)13:8<322:DOIPAI>2.0.ZU;2-S
Abstract
The recently developed whole-column imaged capillary isoelectric focusing f ocusing is an advanced technique for the pI determination of proteins. In t his study, we initially applied this technique to determine the pI of peanu t allergens and its antibody, rabbit IgG. Two main proteins, Ara h I and Ar a h II, were found in the peanut allergen solution. Their pI values were de termined to be about 4.5-4.7 and 5.1-5.6, respectively. The value of pI for the antibody rabbit IgG was 6.1-6.5. Second, the immunoreaction selectivit y of the two main peanut allergenic proteins with antibody rabbit IgG was s tudied. The CIEF results show that the peanut allergen Ara h II has a large r affinity for binding to the antibody rabbit IgG than Ara h I. (C) 2001 Jo hn Wiley & Sons, Inc.