Glutamine transport in C6 glioma cells: Substrate specificity and modulation in a glutamine deprived culture medium

A previous study has shown that glutamine (Gin) uptake in C6 cells grown in a standard medium containing 2 mM Gin, is predominantly mediated by a sodi um-dependent system that is inhibited by ASC system substrates alanine (Ala ), serine (Ser), cysteine (Cys) and threonine (Thr), shows pH sensitivity a nd partial tolerance to substitution of Na+ by Li+, features compatible wit h system ASCT2 that is strongly expressed in cultured astrocytes. The uptak e was not inhibited by the model system A substrate alpha-(methylamino)isob utyric acid (MeAiB), and glycine (Gly) or proline (Pro), indicating that th e substrate-regulated system A as defined by routine criteria is relatively inactive in these cells (Dolinska et al., 2000). In this study we compared the uptake of radiolabeled Gin and a model ASC substrate -Thr in cells gro wn to the same density in Gln-containing and Gln-deprived media. Cells grow n in the absence of Gin showed a reduced activity of system ASC-mediated Gi n uptake, and the system lost tolerance for Li+ and became somewhat more re sistant to lowering pH of the medium. In contrast to cultured astrocytes de prived of Gin, the overall Gin uptake activity in C6 cells adapted to grow in a medium without Gin was lower than in cells grown in a Gin containing m edium, and the uptake by system A remained inactive. C6 cells cultured both in the presence and absence of Gin expressed ASCT2 mRNA, indicating that s ystem ASCT2-mediated Gin uptake is modulated at a posttranscriptional level . In contrast to Gin uptake, Thr uptake was more active in cells cultured i n the absence of Gin and showed neither pH dependence nor lithium tolerance in either medium, which is typical of an uptake mediated by the widespread ASCT1 isoform of system ASC. In C6 cells grown in the presence or absence of Gin alike, approximate to 20% of the sodium-dependent Gin uptake was res istant to MeAiB+Thr, indicating contribution of system N. The N system-medi ated uptake in C6 cells grown in the absence, but not in the presence of Gi n was not inhibited by glutamate (Glu) that conforms to the characteristics of the glial N system variant, SN1. (C) 2001 Wiley-Liss, Inc.