Hd. Je et al., Calponin is required for agonist-induced signal transduction - evidence from an antisense approach in ferret smooth muscle, J PHYSL LON, 537(2), 2001, pp. 567-577
1. The present study was undertaken to determine whether calponin (CaP) par
ticipates in the regulation of vascular smooth muscle contraction and, if s
o, to investigate the mechanism.
2. By PCR homology cloning, the cDNA sequence of ferret basic (h1) CaP was
determined and phosphorothioate antisense and random oligonucleotides were
synthesized and introduced into strips of ferret aorta by a chemical loadin
g procedure.
3. Treatment of ferret aorta with CaP antisense oligonucleotides resulted i
n a decrease in protein levels of CaP to 54% of that in random sequence-loa
ded muscles, but no change in the protein levels of caldesmon (CaD), actin,
desmin or extracellular regulated protein kinase (ERK).
4. Contraction in response to phenylephrine or a phorbol ester was signific
antly decreased in antisense-treated muscles compared to random sequence-lo
aded controls, Neither basal intrinsic tone nor the contraction in response
to 51 mM KCl was significantly affected by antisense treatment.
5. During phenylephrine contractions, phospho-ERK levels increased, as did
myosin light chain (LC20) phosphorylation. Phenylephrine-induced ERK phosph
orylation and CaD phosphorylation at an ERK site were significantly decreas
ed by CaP antisense. Increases in myosin light chain phosphorylation were u
naffected.
6. The data indicate that CaP plays a significant role in the regulation of
contraction and suggest that in a tonically active smooth muscle CaP may f
unction as a signalling protein to facilitate ERK-dependent signalling, but
not as a direct regulator of actomyosin interactions at the myofilament le
vel.