Detection, distribution and selection of microsatellites (SSRs) in the genome of the yeast Saccharomyces cerevisiae as molecular markers

Aims: The aim of this work was the selection of six polymorphic microsatell ite loci for their use as molecular markers in the identification, typifica tion and genetic differentiation of S. cerevisiae strains. Methods and Results: The selection was undertaken following a search of the genomic DNA database of Saccharomyces cerevisiae for simple tandem repeat sequences (microsatellites) of di- and trinucleotides. The genetic variabil ity generated by these markers was evaluated in 51 isolates. The discrimina tory power produced by combining the information obtained by the six micros atellites was very high. A total of 57 alleles, which generated 44 genotype s, were found. Conclusions, Significance and Impact of the Study: The multiple analysis of microsatellites proved to be a powerful and agile tool for analysing the g enome of S. cerevisiae populations.