STATIONARY-PHASE GENE-EXPRESSION IN SACCHAROMYCES-CEREVISIAE DURING WINE FERMENTATION

Citation
C. Riou et al., STATIONARY-PHASE GENE-EXPRESSION IN SACCHAROMYCES-CEREVISIAE DURING WINE FERMENTATION, Yeast, 13(10), 1997, pp. 903-915
Citations number
54
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology",Biology
Journal title
YeastACNP
ISSN journal
0749503X
Volume
13
Issue
10
Year of publication
1997
Pages
903 - 915
Database
ISI
SICI code
0749-503X(1997)13:10<903:SGISDW>2.0.ZU;2-G
Abstract
Genetic engineering of wine yeast strains requires the identification of gene promoters specifically activated under wine processing conditi ons. In this study, transcriptional activation of specific genes was f ollowed during the time course of wine fermentation by quantifying mRN A levels in a haploid wine strain of Saccharomyces cerevisiae grown on synthetic or natural winery musts. Northern analyses were performed u sing radioactive probes from 19 genes previously described as being ex pressed under laboratory growth conditions or on molasses in S. cerevi siae during the stationary phase and/or under nitrogen starvation. Nin e genes, including members of the HSP family, showed a transition-phas e induction profile. For three of them, mRNA transcripts could be dete cted until the end of the fermentation. Expression of one of these gen es, HSP30, was further studied using a HSP30::lacZ fusion on both mult icopy and monocopy expression vectors. The production of beta-galactos idase by recombinant cells was measured during cell growth and ferment ation on synthetic and natural winery musts. We showed that the HSP30 promoter can induce high gene expression during late stationary phase and remains active until the end of the wine fermentation process. Sim ilar expression profiles were obtained on five natural winery musts. ( C) 1997 by John Wiley & Sons, Ltd.