Caspase-3 activation has been implicated in ischemic neuronal death. In the
present study, we examined if cerebral ischemic tolerance induced by suble
thal ischemia is associated with an attenuation of caspase-3 activation in
a mouse forebrain ischemia model. Forebrain ischemia in C57Black/Crj6 strai
n mice was induced by bilateral common carotid artery occlusion (BCCAO) for
18 min. Two episodes of 6-min ischemia were carried out as preconditioning
48 and 72 h before the 18-min BCCAO. Caspase-3-like activity was determine
d by fluorescently monitoring the release of amino-4-methylcoumarin from N-
acetyl-Asp-Glu-Val-Asp-7-amino-4-methylcoumarin in the striatal protein ext
racts at 4, 24, and 72 h after reperfusion. The results showed that the isc
hemic preconditioning significantly attenuated caspase-3 activation at 4, 2
4, and 72 h after reperfusion, and reduced neuronal loss caused by the 18-m
in ischemia as examined on the 7th day after reperfusion. The present resul
ts suggest that the neuroprotection achieved by ischemic preconditioning is
related to an attenuation of caspase-3 activation. (C) 2001 Elsevier Scien
ce Ltd. All rights reserved.