Ci. Keeling et De. Nelson, Changes in the intramolecular stable carbon isotope ratios with age of theEuropean cave bear (Ursus spelaeus), OECOLOGIA, 127(4), 2001, pp. 495-500
Changes with age in the diet and metabolism of the extinct European cave be
ar (Ursus spelaeus) from the Divje Babe archaeological site in northwestern
Slovenia were investigated by comparing the stable carbon isotope ratio of
whole bone collagen with that of the peptide-bonded carboxyl carbons in co
llagen. These carboxyl carbons were selectively released by decarboxylation
of the collagen hydrolysate with ninhydrin (2,2-dihydroxy-1,3-indanedione)
. The stable carbon isotope ratio increased with age for both the peptide-b
onded and other carbons. Although only one-third of the carbon atoms in col
lagen are peptide-bonded, they account for most of the observed change with
age of the stable carbon isotope ratio of the whole collagen. Stable nitro
gen isotope ratios decreased with age and were negatively correlated with c
hanges in the stable carbon isotope ratios. These observations support prev
ious investigations on the unique metabolism of hibernating bears.