Quenching of biotinylated aequorin bioluminescence by dye-labeled avidin conjugates: Application to homogeneous bioluminescence resonance energy transfer assays

Authors
Adamczyk, M Moore, JA Shreder, K
Citation
M. Adamczyk et al., Quenching of biotinylated aequorin bioluminescence by dye-labeled avidin conjugates: Application to homogeneous bioluminescence resonance energy transfer assays, ORG LETT, 3(12), 2001, pp. 1797-1800
Citations number
24
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
ORGANIC LETTERS
ISSN journal
15237060 → ACNP
Volume
3
Issue
12
Year of publication
2001
Pages
1797 - 1800
Database
ISI
SICI code
1523-7060(20010614)3:12<1797:QOBABB>2.0.ZU;2-0
Abstract
[GRAPHICS] Avidin conjugates containing the covalently attached dyes QSY-7 and dabcyl were prepared and shown to quench the bioluminescence of biotinylated aequo rin. Quenching efficiency was shown to be dependent on both the label to av idin ratio and the concentration of the avidin conjugate, These properties were exploited to develop a homogeneous bioluminescence resonance energy tr ansfer (BRET) assay for biotin.