A METHOD FOR PREPARATION OF MAGNETIZABLE CELLULOSE AND ITS APPLICATION IN RADIOIMMUNOASSAYS FOR T-3 AND T-4(1)

An easy adoptable technique has been developed to prepare magnetizable cellulose by the process of wet grinding of a mixture of iron oxide ( magnetite) and cellulose in a stirred ball mill. Anti rabbit IgG was c ovalently coupled to this magnetizable cellulose. The immunosorbent th us prepared has been used in radioimmunoassays of T-3 and T-4 for the separation of bound and free antigens. The reliability of the above im munosorbent has been validated by studying assay parameters such as no n-specific binding (NSB), maximum binding (B-0), CV etc. The single st ep process of wet grinding adopted here not only firmly binds magnetit e and cellulose physically, but also lowers the size of the resulting magnetizable cellulose. The conventional method of preparation involve s the coprecipitation of cellulose dissolved in cuprammonium hydroxide solution along with iron oxide, followed by extensive grinding and si eving of the iron oxide impregnated cellulose.