In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes

Induction of haploid zygotic embryogenesis and androgenesis was studied in 72 and 31 wheat genotypes, respectively. Green zygotic plants (haploids onl y) were obtained in 52 genotypes (72%), and green androgenic regenerants (h aploids and spontaneous polyhaploids) were observed in 17 genotypes (55%). in zygotic embryogenesis embryos developed in 65 genotypes (90%), and in an drogenesis embryos or microsporial calli were detected in 28 genotypes (90% ). The highest frequency of haploid zygotic regenerants was 0.24 per pollin ated flower, whilst that of androgenic regenerants was 0.08 per cultured an ther (0.24 per three anthers in one flower). On average, in induced androge nesis the plants were produced by one out of three androgenic anthers. In i nduced haploid zygotic embryogenesis the plants were regenerated by every t hird flower (ovary) with induced embryos. In a set of selected genotypes th e yield of induced haploid zygotic embryogenesis was compared with induced pollen embryogenesis. The productivity of both methods is discussed on the level of single gametes and in total.