A protective cocktail vaccine against murine cutaneous leishmaniasis with DNA encoding cysteine proteinases of Leishmania major

The protection elicited by the intramuscular injection of two plasmid DNAs encoding Leishmania major cysteine proteinase type I (CPb) and type II (CPa ) was evaluated in a murine model of experimental cutaneous leishmaniasis. BALB/c mice were immunized either separately or with a cocktail of the two plasmids expressing CPa or CPb. II was only when the cpn and cph genes were co-injected that long lasting protection against parasite challenge was ac hieved. Similar protection was also observed when animals were first immuni zed with cpa/cph DNA followed by recombinant CPa/CPb boost. Analysis of the immune response showed that protected animals developed a specific Th1 imm une response, which was associated with an increase of IFN-gamma production . This is the first report demonstrating that co-injection of two genes exp ressing different antigens induces a long lasting protective response, wher eas the separate injection of cysteine proteases genes is not protective. ( C) 2001 Published by Elsevier Science Ltd.