Ja. Tree et al., Comparison of large-scale mammalian cell culture systems with egg culture for the production of influenza virus A vaccine strains, VACCINE, 19(25-26), 2001, pp. 3444-3450
Different types of microcarriers were assessed for the large-scale culture
of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both poro
us and solid carriers were examined. A higher titre of influenza A/PR8/34 v
irus was recovered from cultures using solid (1.3 x 10(9) PFU per ml) rathe
r than porous carriers (4.0 x 10(8) PFU per ml). High titres of virus (1.0
x 10(9) PFU per ml) were also obtained from roller bottle cultures of MDCK
cells and the traditional culture technique using embryonated hens eggs (3.
9 x 10(9) PFU per ml). We found that solid carriers composed of dextran wit
h a positive charge are the most suitable carriers for the large-scale grow
th of influenza A virus in MDCK cells using serum-fret media. Groan Copyrig
ht (C) 2001 Published by Elsevier Science Ltd. All rights reserved.