Modelling the separation of amines by high performance liquid chromatography - I. Linear diamines NH2(CH2)(n)NH2 (n=2-10)

This paper describes the results of a study on the HPLC retention behaviour of general formula NH2(CH2)(n)NH2 (n = 2-10), linear diamines using strong cation exchange resin and amperometric detector. A number of eluents were used for this purpose: citric acid-Na2SO4, H2SO4-Na2SO4, HCl-NaCl, HClO4-Na ClO4 and HClO4-NaClO4-CH3CN. The last eluent ensures smaller retention time s (t(R)) The influence of the anion on t(R) values has been discussed. Empi rical relationships between t(R) and capacity factors K' with Na+ concentra tions and with aliphatic chain length, in different experimental conditions , are reported. The influence of amine protonation constants on log K' valu es is also discussed. Some examples of separation are reported. (C) 2001 El sevier Science B.V. All rights reserved.