Correlation of the near-infrared spectroscopy signals with signal intensity in T-2-weighted magnetic resonance imaging of the human masseter muscle

The purpose of this study was to compare and contrast blood volume changes transcutaneously measured using near-infrared (NIR) spectroscopy against wa ter signal intensity changes taken from a transverse T-2-weighted MR image of the masseter muscle in healthy human subjects before, during and after c ontraction. Eight healthy non-smoking males with no history of chronic musc le pain or vascular headaches participated (mean age: 23.9 +/- 0.6 years). The MRI data were gathered using a turbo spin echo sequence (TR: 2300 ms; T E: 90 ms; FOV: 188 x 300 mm; scanning time: 30 s; slice thickness: 10 mm) a nd the slice level was set at the mid-point between the origin and insertio n of the masseter. Intramuscular haemoglobin (Hb) levels and water content of the right masseter muscle were continuously monitored for 2 min before, 30 s during and 15 min after a maximum voluntary clenching (MVC) task. Both the near-infrared and MRI data were baseline-corrected and normalized and mean levels were established and plotted. Plots of the data showed that bot h near-infrared-based total Hb and T-2-weighted MRI-based signal-intensity levels clearly decreased during contraction and a clear post-contraction re bound response was evident after the contraction. The near-infrared data we re found to be highly correlated with MRI-based signal-intensity data (Pear son's r = 0.909, P < 0.0001). In conclusion, these data provide powerful ev idence that near-infrared data (total Hb), transcutaneously taken from the masseter muscle in humans, will reflect the intramuscular water signal inte nsity changes seen using a T-2-weighted MRI imaging method. (C) 2001 Elsevi er Science Ltd. All rights reserved.