Erythromycin biosynthesis. The 4-pro-S hydride of NADPH is utilized for ketoreduction by both module 5 and module 6 of the 6-deoxyerythronolide B synthase

Authors
Yin, YF Gokhale, R Khosla, C Cane, DE
Citation
Yf. Yin et al., Erythromycin biosynthesis. The 4-pro-S hydride of NADPH is utilized for ketoreduction by both module 5 and module 6 of the 6-deoxyerythronolide B synthase, BIOORG MED, 11(12), 2001, pp. 1477-1479
Citations number
13
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
11
Issue
12
Year of publication
2001
Pages
1477 - 1479
Database
ISI
SICI code
0960-894X(20010618)11:12<1477:EBT4HO>2.0.ZU;2-E
Abstract
Incubation of chirally deuterated NADPH with 6-deoxyerythronolide B synthas e (DEBS) modules 5 and module 6 and analysis of the derived triketide lacto nes established that the two ketoreductase domains, KR5 and KR6, are both s pecific for the 4-pro-S hydride of the nicotinamide cofactor. (C) 2001 Else vier Science Ltd. All rights reserved.