Software scripts for quality checking of high-throughput nucleic acid sequencers

We have developed a graphical interface to allow the researcher to view and assess the quality of sequencing results using a series of program scripts developed to process data generated by automated sequencers. The scripts a re written in Perl programming language and are executable under the cgi-bi n directory of a Web server environment. The scripts direct nucleic acid se quencing trace file data output from automated sequencers to be analyzed by the phred molecular biology program and are displayed as graphical hyperte xt mark-up language (HTML) pages. The scripts are mainly designed to handle 96-well microtiter dish samples, but the scripts are also able to read dat a from 384-well microtiter dishes 96 samples ara time. The scripts may be c ustomized for different laboratory environments and computer configurations . Web links to the sources and discussion page are provided.