W. Min et al., Characterization of recombinant scFv antibody reactive with an apical antigen of Eimeria acervulina, BIOTECH LET, 23(12), 2001, pp. 949-955
The chicken monoclonal antibody (mAb), 6D-12-G10, reacts with an apical com
plex protein at the anterior tip of E. acervulina sporozoites that inhibits
parasite invasion in vitro. Because this mAb was produced at low amount fr
om the original hybridoma cells, an scFv antibody was constructed by amplif
ication of the corresponding V-H and V-L genes and expressed in E. coli. Th
e scFv antibody was produced at a minimum of 7 mg l(-1) and exhibited virtu
ally identical antigen reactivity as the original mAb.