O. Chereguini et al., SHORT-TERM STORAGE AND CRYOPRESERVATION OF TURBOT (SCOPHTHALMUS-MAXIMUS) SPERM, Aquatic living resources, 10(4), 1997, pp. 251-255
Short-term storage over several days as well as cryopreservation of tu
rbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer
200 and artificial seminal liquid (ASL2), are suggested for semen coll
ection in order to avoid the deleterious effect of urine contamination
, and for the purpose of short-term storage between 0 and 15 degrees C
. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot s
permatozoa undergo cryopreservation with a high rate of success especi
ally in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10%
egg yolk.