HISTOCHEMICAL HISTOENZYMIC STUDIES IN BROILER CHICKS FED AFLATOXIN, OCHRATOXIN AND INOCULATED WITH INCLUSION-BODY HEPATITIS-VIRUS SINGLY AND IN CONCURRENCE

The effect of feeding mycotoxins, i.e. aflatoxin B-1 (1.25 ppm from 3 to 38 days of age) and ochratoxin A (0.5 ppm from 3 to 38 days of age) along with inclusion body hepatitis virus (IBHV) inoculation (at 10 d ays of age) singly and in combination was studied in broiler chicks. B irds in combined treatment groups, i.e. aflatoxin fed and virus inocul ated and ochratoxin fed and virus inoculated, showed more changes in a ctivities of phosphatases (AKPase, ATPase, G-6-Pase and ACPase) in liv er and kidney tissues than their respective individual treatment group s with a few exceptions. Reduction in the activities of oxido-reductas es in liver and kidney tissues were almost comparable in different tre atment groups. The increase in muco-polysaccharides reaction was more marked in both the combined treatment groups than the single treatment groups. Intensity of lipid reaction was more in ochratoxin virus comb ination group than either alone.