Allelotype of the adenoma-carcinoma sequence of the stomach

In order to identify the significant allelic loss involving the gastric ade noma-carcinoma sequence, we used 49 genome-wide microsatellite markers in a n allelotype study of 30 cases of stomach resections that harbored both ade nomas and carcinomas. Frequent loss of heterozygosity was demonstrated on 1 2q (53.3%), 2p (50.0%), and 18p (50.0%) in adenomas and on 8q (80.0%), 2p ( 70.0%), 18p (66.7%), and 17p (61.9%) in carcinomas. Significant difference in the loss of heterozygosity rate between the adenoma and the carcinoma wa s noted on 17p. Our results suggested that the critical target of loss of h eterozygosity in gastric adenoma-carcinoma sequences may be the p53 gene on 17p.