Cm. Baker et Hy. Wetzstein, INFLUENCE OF AUXIN TYPE AND CONCENTRATION ON PEANUT SOMATIC EMBRYOGENESIS, Plant cell, tissue and organ culture, 36(3), 1994, pp. 361-368
Somatic embryogenesis in peanut (Arachis hypogaea L.) using immature c
otyledonary explants was induced on a wide range of 2,4-dichlorophenox
yacetic acid (2,4-D) (5 to 60 mg l(-1)) and naphthaleneacetic acid (NA
A) (20 to 50 mg l(-1)) levels. Percent embryogenesis ranged from 31 to
94%. As auxin level increased in induction medium, percent embryogene
sis decreased and was associated with browning of explants. However, w
ith higher 2,4-D induction levels (40 mg l(-1) and over), embryogenic
explants had dense masses of embryogenic areas and repetitive embryoge
nesis was enhanced. Higher auxin concentrations during induction decre
ased precocious germination of embryos, but had no marked effect on so
matic embryo morphology. The use of 2,4-D compared to NAA in the induc
tion medium resulted in greater per cent embryogenesis and mean number
of embryos. Embryos induced on NAA were harder, less pliant, and less
succulent; cultures exhibited more extensive root development and non
embryogenic callus proliferation.