Adenohypophysial cell types in the lamprey pituitary: current state of theart

Citation
M. Nozaki et al., Adenohypophysial cell types in the lamprey pituitary: current state of theart, COMP BIOC B, 129(2-3), 2001, pp. 303-309
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN journal
10964959 → ACNP
Volume
129
Issue
2-3
Year of publication
2001
Pages
303 - 309
Database
ISI
SICI code
1096-4959(200106)129:2-3<303:ACTITL>2.0.ZU;2-6
Abstract
Adenohypophysial cell types in the pituitary of adult sea lampreys, Petromy zon marinus, was localized by means of immunocytochemical and lectin cytoch emical techniques. At least four types of adenohypophysial hormone cells ar e present in the pituitary of adult sea lampreys. The first type of cell is ACTH-like and occupies most parts of the rostral pars distalis (RPD), but a few scattered ACTH-like cells are also present in the proximal pars dista lis (PPD). The second type of cell is MSH-like and occupies the whole pars intermedia. The third type of cell is GH/PRL-like and occupies the dorsal h alf of the PPD. These GH/PRL-like cells were initially detected by heterolo gous immunocytochemistry using antibodies to salmon GH, salmon PRL and blue shark GH, after hydrated autoclave pretreatment of sections. Later, by use of an antiserum raised against a synthetic peptide corresponding to the pa rtial sequence of lamprey GH/PRL, the same cells as those containing GH/PRL -like immunoreactivity were stained positively. Similarity of the topograph ic distributions between lamprey GH/PRL-like cells and gnathostome fish GH cells in the pituitary suggests that GH/PRL-like cells in the lamprey may b e GH cells. The last type of cell is GTH-Iike and occupies the Ventral half of the PPD. Although GTH has not yet been isolated from the lamprey pituit ary, our immunocytochemical data suggest that GTH-like material in the sea lamprey pituitary is more closely related to mammalian-like LH, rather than to FSH or TSH. These four types of adenohypophysial cells occupy most part s of the lamprey adenohypophysis and indeed there is little room for TSH or PRL cells. Thus, the present study further suggests that GH and LH-like GT H are ancestral forms of GH/PRL/SL family and glycoprotein hormones, respec tively. (C) 2001 Elsevier Science Inc. All rights reserved.