Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana
Bd. Rodgers et Gm. Weber, Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana, COMP BIOC B, 129(2-3), 2001, pp. 597-603
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth and
development by inhibiting myoblast proliferation. Mice and cattle possessin
g mutant MSTN alleles display a 'double muscling' phenotype characterized b
y extreme skeletal muscle hypertrophy and/or hyperplasia. MSTN orthologues
have been previously characterized in 12 vertebrate species, including the
white bass Morone chrysops. Presented herein is the identification and char
acterization of novel cDNA clones from two additional Morone species: saxat
ilis (striped bass) and americana (white perch), which were obtained by PCR
amplification and subsequent TA-cloning. The predicted amino acid sequence
of each cDNA clone contains a putative signal sequence, conserved cysteine
residues and a RXXR proteolytic processing site. The different Morone prot
eins were 97-99% identical to each other and approximately 91, 81, 68 and 6
7% identical to the tilapia, zebrafish, mammalian and avian proteins, respe
ctively. However, the bioactive domains, which lie downstream of each proce
ssing site, were considerably more conserved. They were 99-100% identical w
ithin the genus and were approximately 99, 95, 88 and 88% identical to the
tilapia, zebrafish, mammalian and avian domains, respectively. This high le
vel of sequence conservation among all known MSTN orthologues suggests that
the structure/function relationship of each is equally well conserved amon
g vertebrates. (C) 2001 Elsevier Science Inc. All rights reserved.