Nitric oxide production by macrophages of dogs vaccinated with killed Leishmania infantum promastigotes

Human visceral leishmaniosis is endemic in Southern Italy. where the dog is the main reservoir of viscerotropic strains of Leishmania infantum. The re lease of nitric oxide (NO) by interferon (IFN)-gamma -activated macrophages is an important leishmanicidal mechanism in several animal species. In thi s work NO production, phagocytosis and killing capacity of monocyte-derived dog macrophages were evaluated in vitro before and after administration of a vaccine composed of killed Leishmania infantum promastigotes. Moreover, IFN-gamma content was measured in concanavalin A-activated dog peripheral b lood mononuclear cell (PBMC) supernatants employed for macrophage stimulati on. Phagocytosis, killing capacity and NO production by canine macrophages increased significantly 1 month after vaccine administration, and the incre ase also persisted 5 months later. In addition, the amount of IFN-gamma in PBMC supernatants was significantly higher after vaccination. Overall, our results suggest the usefulness of evaluating the in vivo protective role of this promastigote preparation in dogs. (C) 2001 Elsevier Science Ltd. All rights reserved.