Analysis of IgG response to experimental infection with RH Toxoplasma gondii in goats

The IgG response of goats experimentally infected with RH Toxoplasma gondii has been analysed using an indirect ELISA and Western-blot analysis. Speci fic IgG antibodies were first detected at 14 days post-inoculation (p.i.), reaching a peak by day 35 p.i. and showing slight fluctuations until the en d of the experiment (91 p.i.). Specific IgG showed a reactivity over a whol e range of peptides (125-24 kDa approximately). but the highest reactivity was observed against a group of antigens with a molecular weight between 34 and 28 kDa, in particular against a 30 E;Da fraction which is considered t o represent the major surface protein of T. gondii named p30 or SAG-1. (C) 2001 Elsevier Science Ltd. All rights reserved.