NMR methodology for the study of nucleic acids

During the past few years, NMR methodology for the study of nucleic acids h as benefited from new developments that greatly improved state-of-the-art t echnology for the precise determination of three-dimensional structures. Su bstantial progress has been made in designing experimental protocols for th e measurement of residual dipolar couplings, in sensitivity optimization of triple-resonance experiments and in detection of hydrogen bonds and in dev eloping computational methods for structure refinement using NMR restraints .