CLOSE CORRELATION BETWEEN THE DEPHOSPHORYLATION OF P53 AND GROWTH SUPPRESSION BY TRANSFORMING GROWTH-FACTOR-BETA-1 IN NASOPHARYNGEAL CARCINOMA-CELLS TRANSDUCED WITH ADENOVIRUS EARLY REGION GENES

Authors
MOGI Y KATO J HORIMOTO M TAKIMOTO R MURAKAMI T HIRAYAMA A KOHGO Y WATANABE N NIITSU Y
Citation
Y. Mogi et al., CLOSE CORRELATION BETWEEN THE DEPHOSPHORYLATION OF P53 AND GROWTH SUPPRESSION BY TRANSFORMING GROWTH-FACTOR-BETA-1 IN NASOPHARYNGEAL CARCINOMA-CELLS TRANSDUCED WITH ADENOVIRUS EARLY REGION GENES, Japanese journal of cancer research, 85(5), 1994, pp. 459-463
Citations number
29
Categorie Soggetti
Oncology
Journal title
Japanese journal of cancer research → ACNP
ISSN journal
09105050
Volume
85
Issue
5
Year of publication
1994
Pages
459 - 463
Database
ISI
SICI code
0910-5050(1994)85:5<459:CCBTDO>2.0.ZU;2-Z
Abstract
The mechanism of growth inhibition by transforming growth factor (TGF) ;beta 1 was investigated. We examined the growth inhibitory effects of TGF-beta 1 an human nasopharyngeal carcinoma (KB) cells which constit utively expressed p53. TGF-beta 1 suppressed the DNA synthesis of KB c ells in a dose-dependent manner. Tt had minimal effect on adenovirus-2 -transduced I(B cells expressing either adenovirus early region 1B (E1 B) or 1A (EIA) product, which respectively binds to p53 or Rb product and inhibits its function, and no growth inhibition at all was observe d with KB cells expressing both E1B and E1A products. Dephosphorylatio n of the p53 was promoted by TGF-beta 1 stimulation in KB cells, but n ot in E1B-producing I(B cells, which sequestrate the function of p53. The growth inhibition of KB cells by TGF;beta 1 was significantly redu ced by treatment with okadaic acid. These results suggest that p53 tra nsduces the antiproliferative signal of TGF;beta 1 possibly through it s dephosphorylation.