Luteolin-inhibited arylamine N-acetyltransferase activity and DNA-2-aminofluorene adduct in human and mouse leukemia cells

N-Acetyltransferase enzyme is an important enzyme in the first step of aryl amine compounds metabolism. Luteolin has been shown to exit antibacterial a nd antineoplastic activity. The purpose of this present study is to evaluat e the question of whether luteolin could affect arylamine N-acetyltransfera se (NAT) activity and DNA-2-aminofluorene adduct formation in human (HL-60) and mouse (L1210) leukemia cells. By using HPLC, N-acetylation of 2-aminof luorene wase determined. Luteolin displayed a dose-dependent inhibition to cytosolic NAT activity and intact human and mice leukemia cells. Time-cours e experiments showed that N-acetylation or 2-aminofluorene measured from in tact human and mice leukemia cells were inhibited by luteolin for up to 24 hours. Using standard steady-state kinetic analysis, it was demonstrated th at luteolin was a possible uncompetitive inhibitor to NAT activity in cytos ols. The DNA-2-aminofluorene adduct formation in human and mouse leukemia c ells were inhibited by luteolin. This report is the first demonstration to show that luteolin affects human and mice leukemia cells NAT activity and D NA-2-aminofluorene on adduct formation. (C) 2001 Elsevier Science Ltd. All rights reserved.