Characterization of digestive carbohydrase activity in the gilthead seabream (Sparus aurata)

Authors
Alarcon, FJ Martinez, TF Diaz, M Moyano, FJ
Citation
Fj. Alarcon et al., Characterization of digestive carbohydrase activity in the gilthead seabream (Sparus aurata), HYDROBIOL, 445(1-3), 2001, pp. 199-204
Citations number
30
Categorie Soggetti
Aquatic Sciences
Journal title
HYDROBIOLOGIA
ISSN journal
00188158 → ACNP
Volume
445
Issue
1-3
Year of publication
2001
Pages
199 - 204
Database
ISI
SICI code
0018-8158(2001)445:1-3<199:CODCAI>2.0.ZU;2-C
Abstract
The presence of digestive carbohydrases was determined in seabream intestin es, and the main activity identified was amylase. Optimum activity for this enzyme was found at pH 8.0 at 40 degreesC. Amylase activity was highly sen sitive to extreme pH, and temperatures exceeding 50 degreesC. The use of SD S-PAGE zymograms allowed identification of amylase in the form of a high mo lecular mass fraction exceeding 100 kDa. Results confirm the existence of a well developed amylase equipment in this species which supports the possib ility of increasing the amount of carbohydrates in the formulation of its c ommercial feeds.