The clinical relevance of microbiologic testing: A comparative analysis ofmicrobiologic samples secured from the same sites and cultured in two independent laboratories
Jr. Mellado et al., The clinical relevance of microbiologic testing: A comparative analysis ofmicrobiologic samples secured from the same sites and cultured in two independent laboratories, INT J PER R, 21(3), 2001, pp. 233-239
Citations number
11
Categorie Soggetti
Dentistry/Oral Surgery & Medicine
Journal title
INTERNATIONAL JOURNAL OF PERIODONTICS & RESTORATIVE DENTISTRY
A field study using five different private periodontal practices was conduc
ted; it compared two microbiologic culture samples simultaneously secured f
rom the same sites within 23 individual patients and submitted for bacteria
l identification and antibiotic sensitivity testing to two separate laborat
ories. The results from the two laboratories were often different. In no in
stance did both laboratories agree on the presence of identical bacterial s
pecies. When only bacteria above threshold levels were compared, agreement
was found in only nine of 23 cases. When examining antibiotic sensitivity,
using 100% kill of all tested pathogens as the ideal, agreement between the
two laboratories was poor. The laboratories agreed on the use of amoxicill
in 17% of the time, tetracycline 26% of the time, and metronidazole 48% of
the time. The use of amoxicillin and metronidazole in combination yielded a
78% agreement when the results of both laboratories were combined, it woul
d appear from the data that the empirical use of amoxicillin-metronidazole
combination therapy may be more clinically sound and cost effective than cu
lturing and antibiotic selection based on the results of culture from any s
ingle microbiologic testing laboratory.