Cm. Boone et al., INVESTIGATION OF BEAM-INDUCED REACTIONS OCCURRING UNDER FAST-ATOM-BOMBARDMENT CONDITIONS BETWEEN TRIETHANOLAMINE AND VARIOUS PHOSPHOLIPIDS, Rapid communications in mass spectrometry, 11(11), 1997, pp. 1205-1211
The generation of adduct ions when using a triethanolamine (TEA) matri
x under conditions of fast-atom bombardment has been investigated. in
particular, phospholipids containing a free amino group react in the T
EA matrix to form abundant adduct ions 26 and 42 mass units higher tha
n the analyte pseudomolecular ions, The identity of these adduct ions
was investigated using specific phosphatidylethanolamines and phosphat
idylserine as well as 2-aminoethyldihydrogenphosphate and 2-aminoethyl
monomethylphosphate as model compounds, Evidence obtained from positiv
e- and negative-ion high-energy collision-induced dissociation tandem
mass spectra, high resolution mass spectra, deuterium labelling and ex
periments using diethanolamine/TEA mixtures revealed that the intensit
ies of the adduct ions are dependent on the percentage of TEA in the m
atrix and on the polarity of the analyte. The nature of the adduct ion
s is best reflected by a structure in which a hydrogen atom of the fre
e amino group is substituted by a vinyl (+ 26) or by an acetyl (+ 42)
group, A mechanism for the adduct ion formation is proposed. (C) 1997
by John Wiley & Sons, Ltd.