REPRESENTATION OF CLONED GENOMIC SEQUENCES IN 2 SEQUENCING VECTORS - CORRELATION OF DNA-SEQUENCE AND SUBCLONE DISTRIBUTION

Representation of subcloned Caenorhabditis elegans and human DNA seque nces in both M13 and pUC sequencing vectors was determined in the cont ext of large scale genomic sequencing. In many cases, regions of subcl one under-representation correlated with the occurrence of repeat sequ ences, and in some cases the under-representation was orientation spec ific, Factors which affected subclone representation included the natu re and complexity of the repeat sequence, as well as the length of the repeat region, in some but not ail cases, notable differences between the M13 and pUC subclone distributions existed. However, in all regio ns lacking one type of subclone (either M13 or pUC), an alternate subc lone was identified in at least one orientation. This suggests that co mplementary use of M13 and pUC subclones would provide the most compre hensive subclone coverage of a given genomic sequence.