SYNTHESIS AND HYBRIDIZATION PROPERTIES OF INVERSE OLIGONUCLEOTIDES

The synthesis of adenine and thymine cyclopentylethyl nucleosides is p resented. This novel constrained monomeric building block is very diff icult to incorporate into oligonucleotides. it was introduced in 13mer oligodeoxynucleotide sequences at a single position using H-phosphona te chemistry. Phosphoramidite chemistry completely failed in this part icular case. The H-phosphonate building blocks were obtained starting from the corresponding phosphoramidites, Stability of duplexes with RN A and DNA is significantly reduced.