Radiochemical synthesis and preliminary evaluation of anti-bladder cancer monoclonal antibody BDI-1 labeled with rhenium-188

The anti-human bladder cancer monoclonal antibody BDI-1 was radio-labeled w ith rhenium-188 by direct labeling methods using SnCl2 as reductant and MDP as stannous stabilizer or by indirect method using NHS-ECM ester as bifunc tional chelator. Radiochemical yields of 30 +/- 7.23% and 87.4 +/- 5.67% an d radiochemical purity of more than 95% were achieved. The inmmunoreactive fraction was 58.7%. The results showed that radionuclide Re-188 might be em ployed using the same labeling methodology with Tc-99m labeling of BDI-1 fo r radiommunoimaging (RII) and radioimmunotherapy (RIT) and that may be usef ul in radioimmunodetection and RIT of human bladder carcinoma in vivo. Copy right (C) 2001 John Wiley & Sons, Ltd.