RADIOIODINATED ANTIBODY TARGETING OF THE HER-2 NEU ONCOPROTEIN/

The HER-2/neu oncogene encodes a 185 kDa phosphoglycoprotein that is o verexpressed in breast, ovarian and other cancers. Seven monoclonal an tibodies reactive with this oncoprotein were labeled with I-131. In vi tro experiments with SKOv3 9002-18 cells determined binding affinity, internalization and degradation. The biodistribution of these antibodi es in comparison to I-125-labeled nonspecific antibody was measured in athymic mice with SKOv3 9002-18 ovarian carcinoma xenografts. Antibod y 520C9 exhibited the highest and most specific retention in tumor, pe aking at 17.4 +/- 5.6% ID/g at 24 h. (C) 1997 Elsevier Science Inc.