The microbial contamination of in-use sponges was investigated. Of the sixt
y sponges examined, 51 (85%) were contaminated with 10(3)-10(9) colony form
ing units (CFU) per sponge. Pseudomonas aeruginosa was isolated from sixtee
n (26.7%) of the sixty sponges. P. aeruginosa survived for 2 months in cont
aminated sponges which were left at room temperature and became dry to the
touch. The susceptibility of sponges to P. aeruginosa contamination should
be recognized. Once sponges are contaminated with P. aeruginosa, eradicatio
n of this organism is difficult even if the sponges are dried.