Transformation of Brazilian elite Indica-type rice (Oryza sativa L.) by electroporation of shoot apex explants

Authors
de Padua, VLM Ferreira, RP Meneses, L Uchoa, N Margis-Pinheiro, M Mansur, E
Citation
Vlm. De Padua et al., Transformation of Brazilian elite Indica-type rice (Oryza sativa L.) by electroporation of shoot apex explants, PL MOL B RE, 19(1), 2001, pp. 55-64
Citations number
29
Categorie Soggetti
Plant Sciences
Journal title
PLANT MOLECULAR BIOLOGY REPORTER
ISSN journal
07359640 → ACNP
Volume
19
Issue
1
Year of publication
2001
Pages
55 - 64
Database
ISI
SICI code
0735-9640(200103)19:1<55:TOBEIR>2.0.ZU;2-U
Abstract
We have generated transgenic plants of a Brazilian elite Indica-type rice b y electroporation of shoot apices. This approach avoids a callus phase and produces 0.4-13.8% resistant plants. Transgenic plantlets were transferred to soil a few weeks after explant electroporation. Root segments from plant lets obtained from transformation experiments with pAHC25 plasmid were GUS positive. Integration of the introduced gene into the genome was demonstrat ed by PPT and antibiotic screening as well as by PCR and Southern blot hybr idization of genomic DNA isolated from R-2 plantlets.