Inventory of the superfamily of P-type ion pumps in Arabidopsis

A total of 45 genes encoding for P-type ATPases have been identified in the complete genome sequence of Arabidopsis. Thus, this plant harbors a primar y transport capability not seen in any other eukaryotic organism sequenced so far. The sequences group in all five subfamilies of P-type ATPases. The most prominent subfamilies are P-1B ATPases (heavy metal pumps; seven membe rs), P-2A and P-2B ATPases (Ca2+ pumps; 14 in total), P-3A ATPases (plasma membrane Wi pumps; 12 members including a truncated pump, which might repre sent a pseudogene or an ATPase-like protein with an alternative function), and P, ATPases (12 members). P, ATPases have been implicated in aminophosho lipid flipping but it is not known whether this is a direct or an indirect effect of pump activity. Despite this apparent plethora of pumps, Arabidops is appears to be lacking Na+ pumps and secretory pathway (PMR1-like) Ca2+-A TPases. A cluster of Arabidopsis heavy metal pumps resembles bacterial Zn2/Co2+/Cd2+/Pb2+ transporters. Two members of the cluster have extended C te rmini containing putative heavy metal binding motifs. The complete inventor y of P-type ATPases in Arabidopsis is an important starting point for rever se genetic and physiological approaches aiming at elucidating the biologica l significance of these pumps.