Regeneration of shoot-buds on hypocotyl of Linum seedlings: a stress-related response

Transient stress of different types effective in induction of shoot-buds on hypocotyl of seedlings are heat-, salt- and mineral-stress, ABA- and proli ne-treatment and a simple decapitation. Of these decapitation is known and the rest have been worked out for the first time. Heat- and salt-stress wer e dose dependent, ABA-treatment was effective, in induction of shoot-buds, when it was combined with a prior mineral-stress. In addition to their indi vidual effect in promoting regeneration, there was synergism between differ ent types of stress. Highest frequency of shoot formation occurred when min eral-stress was combined with decapitation. (C) 2001 Elsevier Science Irela nd Ltd. All rights reserved.