Dynamics of two proteins [myoglobin and photoactive yellow protein (PYP)] i
nitiated by photoexcitation were studied by the time-resolved transient gra
ting (TG) and photoacoustic methods. For both proteins, the temporal profil
es of the TG signals showed new features that have not been detected before
by the transient absorption method. These dynamics were attributed to the
volume change, which is caused by the protein dynamics apart from the chrom
ophores.