Background and Objective To validate a standardized optimal national proced
ure for donor arm disinfection.
Materials and Methods A direct swabbing and plating technique was used to e
numerate bacteria present on the arm pre- and postdisinfection. Twelve dono
r arm disinfection techniques were evaluated.
Results The Medi-Flex Adapted method, consisting of a two-stage process wit
h an initial application of isopropyl alcohol followed by tincture of iodin
e, produced the best arm disinfection. A percentage reduction in bacterial
counts of 99.79% (logarithmic reduction of 2.67) was obtained. Postdisinfec
tion, 70% of donors had bacterial counts of zero, and 98% had counts of 10
or less.
Conclusion The Medi-Flex disinfection method offers the English National Bl
ood Service a validated, optimal 'best practice' disinfection technique and
should contribute significantly to the reduction in risk of transmission o
f bacteria by transfusion.