DNA GENOTYPESCOPY IN PATERNITY TESTING - USE OF HYBRIDIZATION PROBES

Analysis of hybridization probes for DNA genotypescopy (DNA genotyping and genome fingerprinting) was performed to detect 21 cases of patern ity testing. A system with the highly informative multilocus DNA probe Red4, isolated by us earlier, and two single-locus probes (YNH24 and CMM101) detecting highly polymorphic (H > 96%) loci D2S44 and D14S13 w as tested. In the cases analyzed, the Red4 probe was shown to detect, on average, 19.28 +/- 3.6 polymorphic BsuRI fragments in the DNA profi le of presumable fathers and 19.67 +/- 5.84 BsuRI fragments in the DNA profile of mothers. The average number of DNA fragments inherited by a child from either parent was approximately equal (8.72 +/- 3.77 and 7.11 +/- 2.66, respectively). The low population frequency of DNA frag ments detected by the Red4 probe allowed highly effective positive pat ernity identification to be performed. Paternity was established in 86 % (with probability >99.75 or >99.99%) and excluded in 14% of expertis es. Single-locus probes YNH24 and CMM101 were used as an additional cr iterion in cases when, in the DNA profile of a child, a single band (p robable de novo mutation) or several bands (probable false paternity o r maternity) were revealed but absent in both presumable parents. In o ne case, a de novo mutation for the YNH24 probe, not described earlier , was revealed. Therefore, a combination of multilocus and single-locu s hybridization probes appeared to be the most promising method for si gnificant paternity testing in forensic and medical genetic practice.