Crystallization and preliminary X-ray crystallographic studies on 4-alpha-glucanotransferase from Thermotoga maritima

Authors
Roujeinikova, A Raasch, C Sedelnikova, S Liebl, W Rice, DW
Citation
A. Roujeinikova et al., Crystallization and preliminary X-ray crystallographic studies on 4-alpha-glucanotransferase from Thermotoga maritima, ACT CRYST D, 57, 2001, pp. 1046-1047
Citations number
17
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
57
Year of publication
2001
Part
7
Pages
1046 - 1047
Database
ISI
SICI code
0907-4449(200107)57:<1046:CAPXCS>2.0.ZU;2-H
Abstract
Thermotoga maritima 4-alpha -glucanotransferase (GTase), a 52 kDa molecular -weight amylolytic enzyme, has been crystallized by the hanging-drop vapour -diffusion method using PEG monomethylether 5000 as a precipitating agent. A complete data set has been collected to 2.6 Angstrom resolution using cry ocooling conditions and synchrotron radiation. The crystals belong to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 92.6, b = 180.3 , c = 199.2 Angstrom.