Crystallization and preliminary crystallographic data of fructose-1,6-bisphosphatase from human muscle

The enzyme human muscle fructose-1,6-bisphosphatase, which plays a critical role in gluconeogenesis, has been crystallized in the presence of 2-propan ol, polyethylene glycol and magnesium chloride at pH 7.5. The space group w as determined to be P4(2)2(1)2, with unit-cell parameters a = b = 73.57, c = 146.50 Angstrom, alpha = beta = lambda = 90 degrees and one subunit in th e asymmetric unit. A 99.6% complete data set to 2.04 Angstrom has been coll ected at the National Synchrotron Light Source.