Crystallization and preliminary X-ray study of haem-binding protein from the bloodsucking insect Rhodnius prolixus

Authors
Nagem, RAP Neto, JRB Forrer, VP Sorgine, MH Paiva-Silva, GO Masuda, H Meneghini, R Oliveira, PL Polikarpov, I
Citation
Rap. Nagem et al., Crystallization and preliminary X-ray study of haem-binding protein from the bloodsucking insect Rhodnius prolixus, ACT CRYST D, 57, 2001, pp. 860-861
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
57
Year of publication
2001
Part
6
Pages
860 - 861
Database
ISI
SICI code
0907-4449(200106)57:<860:CAPXSO>2.0.ZU;2-4
Abstract
Rhodnius haem-binding protein (RHBP) from the bloodsucking insect Rhodnius prolixus, a 15 kDa protein, has been crystallized using polyethylene glycol as a precipitant. X-ray diffraction data have been collected at a synchrot ron source. The crystals belong to the space group P4(1(3))2(1)2, with unit -cell parameters a = b = 64.98, c = 210.68 Angstrom, and diffract beyond 2. 6 Angstrom resolution.