Y. Nakamura et al., Crystallization and preliminary X-ray diffraction analysis of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechococcus PCC 7942, ACT CRYST D, 57, 2001, pp. 879-881
The NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Syne
chococcus PCC 7942 was crystallized in two different forms by the hanging-d
rop vapour-diffusion method using ammonium sulfate as a precipitant. Form I
crystals were hexagonal, space group P6(5) or P6(1), with unit-cell parame
ters a = b = 91.1, c = 428.6 Angstrom, gamma = 120 degrees. Form II crystal
s were monoclinic, space group C2, with unit-cell parameters a = 152.3, b=
80.9, c = 213.6 Angstrom, beta= 103.1 degrees. Native data were collected f
rom a frozen crystal of form I to a resolution of 2.8 Angstrom using synchr
otron radiation at SPring-8, whereas form II crystals were easily damaged b
y radiation at room temperature and increased mosaicity in cryoprotectant s
olutions. A molecular-replacement solution of the form I crystal was obtain
ed in space group P6(5) using the program AMoRe and the structure of the NA
D-dependent GAPDH from Bacillus stearothermophilus.