Cyclin A and Ki-67 with DNA content in benign and malignant prostatic epithelial lesions

OBJECTIVE: To evaluate the usefulness of immunohistochemical staining of cy clin A and Ki-67 together with DNA content in the classification of benign prostatic hyperplasia, prostatic intraepithelial neoplasm (PIN) and prostat ic carcinoma foci and to compare these parameters with each other and with parameters obtained from conventional histopathology. STUDY DESIGN: We selected 37 carcinoma, 18 PIN and 8 hyperplastic foci from prostatectomies clone during 1996 and 1997 at Turku University Central Hos pital. Cyclin A and Ki-67 staining was assessed by immunohistochemistry and DNA content by image cytometry. RESULTS: The hyperplastic, PIN and carcinoma foci differed clearly in their 2.5c exceeding rates, image cytometric proliferation indices and staining indices for cyclin A and Ki-67. No significant differences were found betwe en these histologic entities in their modal DNA ploidy values. III carcinom as, cyclin A and Ki-67 indices differed between low, intermediate and high Gleason and World Health Organization grading groups. Diploid and tetraploi d carcinomas had silmilar cyclin A and Ki-67 indices, which differed from t hose of aneuploid carcinomas. CONCLUSION: The 2.5c exceeding rate and image cytometric proliferation inde x as well as the cyclin A and Ki-67 indices differed significantly between different types of prostatic lesions. Cyclin A and Ki-67 had good correlati ons with the histologic grade of carcinoma.