BIACORE data processing: An evaluation of the global fitting procedure

Data from real-time molecular interaction analysis using BIACORE are curren tly evaluated by numerical integration. We have investigated the ability of two software packages (BLCevaluation 3.0 and CLAMP99) to analyze complex i nteractions. Three experimental data sets of high quality obtained with BIA CORE upgraded and 2000 instruments, representative of simple bimolecular, h eterogeneous ligand, and mass-transport-limited interactions, were processe d by the global fitting procedure. The two software, which differ mainly in the statistical assessment of the output values,:were able to discriminate correctly between various interacting models and provided very close outpu t parameters with satisfactory statistical tests. (C) 2001 Academic Press.