Biochemical mechanism of human immunodeficiency virus type 1 reverse transcriptase resistance to stavudine

Authors
Lennerstrand, J Stammers, DK Larder, BA
Citation
J. Lennerstrand et al., Biochemical mechanism of human immunodeficiency virus type 1 reverse transcriptase resistance to stavudine, ANTIM AG CH, 45(7), 2001, pp. 2144-2146
Citations number
26
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
7
Year of publication
2001
Pages
2144 - 2146
Database
ISI
SICI code
0066-4804(200107)45:7<2144:BMOHIV>2.0.ZU;2-T
Abstract
We have found a close correlation between viral stavudine (d4T) resistance and resistance to d4T-triphosphate at the human immunodeficiency virus type 1 reverse transcriptase (RT) level, RT from site-directed mutants with 69S -XX codon insertions and/or conventional zidovudine resistance mutations se ems to be involved in an ATP-dependent resistance mechanism analogous to py rophosphorolysis, whereas the mechanism for RT with the Q151M or V75T mutat ion appears to be independent of added ATP for reducing binding to d4T-trip hosphate.