Heat treatment of amphotericin B modifies its serum pharmacokinetics, tissue distribution, and renal toxicity following administration of a single intravenous dose to rabbits
Eh. Kwong et al., Heat treatment of amphotericin B modifies its serum pharmacokinetics, tissue distribution, and renal toxicity following administration of a single intravenous dose to rabbits, ANTIM AG CH, 45(7), 2001, pp. 2060-2063
The purpose of this investigation was to determine the serum pharmacokineti
cs, tissue distribution, and renal toxicity of amphotericin B (AmpB) follow
ing administration of a single intravenous dose (1 mg/kg of body weight) of
Fungizone (FZ) and a heat-treated form of FZ (HFZ) to New Zealand White fe
male rabbits. FZ solutions were heated at 70 degreesC for 20 min to produce
HFZ. Blood samples were obtained before drug administration and serially t
hereafter. After collection of the 48-h blood sample, each rabbit was human
ely sacrificed and the right kidney, spleen, lungs, liver, and heart were h
arvested for AmpB analysis. Serum creatinine levels were measured before an
d 10 h after drug administration. AmpB concentrations in the serum and tiss
ues were analyzed using high-performance liquid chromatography. FZ administ
ration to rabbits resulted in a greater-than-50% increase in serum creatini
ne concentrations compared to baseline. However, HFZ administration resulte
d in no difference in serum creatinine concentrations compared to baseline.
The AmpB area under the concentration-time curve (AUC) after HFZ administr
ation was significantly lower than the AmpB AUC in rabbits administered FZ.
However, AmpB systemic total body clearance was significantly greater in r
abbits administered HFZ than in rabbits administered FZ without any differe
nces in volume of distribution at steady state. Kidney tissue AmpB concentr
ations, although not significantly different, were greater in rabbits admin
istered FZ than in rabbits administered HFZ. Likewise, lung and spleen AmpB
concentrations, although not significantly different, were greater in rabb
its administered FZ than in rabbits administered HFZ. However, liver AmpB c
oncentrations were significantly lower in rabbits administered FZ than in r
abbits administered HFZ. No significant differences in heart AmpB concentra
tion between rabbits administered PZ and those given HFZ were found. These
findings suggest that the pharmacokinetics, tissue distribution, and renal
toxicity of AmpB are modified following administration of HFZ, HFZ could be
an improved low-cost AmpB drug delivery system that has a potentially high
er therapeutic index than FZ.